Development of an Enzyme-Linked Immunosorbent Assay for Goldfish Gonadotropin
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چکیده
منابع مشابه
Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملDevelopment of an enzyme - linked immunosorbent assay ( ELISA ) for the
6 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of 7 Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania; Veterinary 8 Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, 9 University of Liège, Liège, Belgium; Dermatology and Venereology Department, Laboratory of 10 Mycology, Centr...
متن کاملEvaluation of Enzyme Linked Immunosorbent Assay, Utilizing Native Antigen B for Serodiagnosis of Human Hydatidosis
Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...
متن کاملenzyme-linked immunosorbent assay
An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1989
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod41.1.68